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h11 locus homology arms  (Addgene inc)


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    Structured Review

    Addgene inc h11 locus homology arms
    H11 Locus Homology Arms, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/homology+arms/PCR4-Shh%3A%3AlacZ-H11+(Plasmid+%23139098)/pm41916278-269-43-47
    Average 94 stars, based on 40 article reviews
    h11 locus homology arms - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: A missense mutation in human INSC causes peripheral neuropathy.
    Article Snippet: To clone the donor DNA plasmid, both upstream and downstream homology arms of dInsc were amplified from fly genomic DNA by Phusion® High-Fidelity DNA Polymerase (New England Biolab #M0530S) under optimized conditions. .. The two homology arms were then cloned into pScarlessHD-DsRed (Addgene #64703) plasmid by SOEing PCR, followed by standard heat-shock transformation, colony PCR selection, and Sanger sequencing. .. Embryo microinjection service was provided by Wellgenetics, Taiwan, to inject the gRNA and donor DNA plasmids into fly embryos with Cas9 activity in the germ cell.

    Article Title: A genome-engineered tool set for Drosophila TGF-β/BMP signaling studies.
    Article Snippet: Ligands of the TGF-β/BMP superfamily are critically involved in the regulation of growth, patterning and organogenesis and can act as long-range morphogens.. Essential for understanding TGF-β/BMP signaling dynamics and regulation are tools that allow monitoring and manipulating pathway components expressed at physiological levels and endogenous spatiotemporal patterns.. We used genome engineering to generate a comprehensive library of endogenously epitopeor fluorescently-tagged versions of receptors, co-receptors, transcription factors and key feedback regulators of the Drosophila BMP and Activin signaling pathways.

    Article Title: Dual regulation of the unfolded protein response by IGF2BP3 during ER stress
    Article Snippet: The IGF2BP3 homology arms were inserted into the pMK344 plasmid backbone (Addgene #121179) using SacI and XhoI restriction sites. .. BSD-P2A-mAID sequence was cloned into the plasmid with N-terminal homology arms based on IGF2BP3 from the pMK347 plasmid (Addgene #121181) using SalI and BamI restriction sites resulting in the HDR template. ..

    Article Title: A genome engineered tool set for Drosophila TGF-β/BMP signaling studies
    Article Snippet: .. PCR-amplified homology arms were cloned into the pHD-dsRed-attP reintegration vector (Addgene #51019 ( )) and guide RNAs were cloned into plasmid pCFD3-dU6:3 (for Dlp, Mad and Med) or pCFD4-U6:1_U6:3 (for Babo, Brk, Dad, Sax, Shn, Smad2 and Wit) (Addgene #49410 and #49411, ( )). ..

    Plasmid Preparation:

    Article Title: A missense mutation in human INSC causes peripheral neuropathy.
    Article Snippet: To clone the donor DNA plasmid, both upstream and downstream homology arms of dInsc were amplified from fly genomic DNA by Phusion® High-Fidelity DNA Polymerase (New England Biolab #M0530S) under optimized conditions. .. The two homology arms were then cloned into pScarlessHD-DsRed (Addgene #64703) plasmid by SOEing PCR, followed by standard heat-shock transformation, colony PCR selection, and Sanger sequencing. .. Embryo microinjection service was provided by Wellgenetics, Taiwan, to inject the gRNA and donor DNA plasmids into fly embryos with Cas9 activity in the germ cell.

    Article Title: A genome-engineered tool set for Drosophila TGF-β/BMP signaling studies.
    Article Snippet: Ligands of the TGF-β/BMP superfamily are critically involved in the regulation of growth, patterning and organogenesis and can act as long-range morphogens.. Essential for understanding TGF-β/BMP signaling dynamics and regulation are tools that allow monitoring and manipulating pathway components expressed at physiological levels and endogenous spatiotemporal patterns.. We used genome engineering to generate a comprehensive library of endogenously epitopeor fluorescently-tagged versions of receptors, co-receptors, transcription factors and key feedback regulators of the Drosophila BMP and Activin signaling pathways.

    Article Title: Dual regulation of the unfolded protein response by IGF2BP3 during ER stress
    Article Snippet: The IGF2BP3 homology arms were inserted into the pMK344 plasmid backbone (Addgene #121179) using SacI and XhoI restriction sites. .. BSD-P2A-mAID sequence was cloned into the plasmid with N-terminal homology arms based on IGF2BP3 from the pMK347 plasmid (Addgene #121181) using SalI and BamI restriction sites resulting in the HDR template. ..

    Article Title: A genome engineered tool set for Drosophila TGF-β/BMP signaling studies
    Article Snippet: .. PCR-amplified homology arms were cloned into the pHD-dsRed-attP reintegration vector (Addgene #51019 ( )) and guide RNAs were cloned into plasmid pCFD3-dU6:3 (for Dlp, Mad and Med) or pCFD4-U6:1_U6:3 (for Babo, Brk, Dad, Sax, Shn, Smad2 and Wit) (Addgene #49410 and #49411, ( )). ..

    Article Title: Transcription processes compete with loop extrusion to homogenize promoter and enhancer dynamics
    Article Snippet: .. PCR amplification and Gibson assembly was used to replace the homology arms of plasmid pCRIS-PITChv2-dTAG-Puro (BRD4) (Addgene, #91796) with those for Rad21 , and 1 μg of the vector was cotransfected with 3 μg of plasmid containing Cas9-GFP, a puromycin-resistance marker and a scaffold to encode the gRNA specific to the insertion site (generated by the IGBMC molecular biology platform; sequence of gRNA given in table S1) in 1 million F1 ESCs with Lipofectamine 2000. ..

    Polymerase Chain Reaction:

    Article Title: A missense mutation in human INSC causes peripheral neuropathy.
    Article Snippet: To clone the donor DNA plasmid, both upstream and downstream homology arms of dInsc were amplified from fly genomic DNA by Phusion® High-Fidelity DNA Polymerase (New England Biolab #M0530S) under optimized conditions. .. The two homology arms were then cloned into pScarlessHD-DsRed (Addgene #64703) plasmid by SOEing PCR, followed by standard heat-shock transformation, colony PCR selection, and Sanger sequencing. .. Embryo microinjection service was provided by Wellgenetics, Taiwan, to inject the gRNA and donor DNA plasmids into fly embryos with Cas9 activity in the germ cell.

    Article Title: A genome-engineered tool set for Drosophila TGF-β/BMP signaling studies.
    Article Snippet: Ligands of the TGF-β/BMP superfamily are critically involved in the regulation of growth, patterning and organogenesis and can act as long-range morphogens.. Essential for understanding TGF-β/BMP signaling dynamics and regulation are tools that allow monitoring and manipulating pathway components expressed at physiological levels and endogenous spatiotemporal patterns.. We used genome engineering to generate a comprehensive library of endogenously epitopeor fluorescently-tagged versions of receptors, co-receptors, transcription factors and key feedback regulators of the Drosophila BMP and Activin signaling pathways.

    Article Title: A genome engineered tool set for Drosophila TGF-β/BMP signaling studies
    Article Snippet: .. PCR-amplified homology arms were cloned into the pHD-dsRed-attP reintegration vector (Addgene #51019 ( )) and guide RNAs were cloned into plasmid pCFD3-dU6:3 (for Dlp, Mad and Med) or pCFD4-U6:1_U6:3 (for Babo, Brk, Dad, Sax, Shn, Smad2 and Wit) (Addgene #49410 and #49411, ( )). ..

    Article Title: Transcription processes compete with loop extrusion to homogenize promoter and enhancer dynamics
    Article Snippet: .. PCR amplification and Gibson assembly was used to replace the homology arms of plasmid pCRIS-PITChv2-dTAG-Puro (BRD4) (Addgene, #91796) with those for Rad21 , and 1 μg of the vector was cotransfected with 3 μg of plasmid containing Cas9-GFP, a puromycin-resistance marker and a scaffold to encode the gRNA specific to the insertion site (generated by the IGBMC molecular biology platform; sequence of gRNA given in table S1) in 1 million F1 ESCs with Lipofectamine 2000. ..

    Transformation Assay:

    Article Title: A missense mutation in human INSC causes peripheral neuropathy.
    Article Snippet: To clone the donor DNA plasmid, both upstream and downstream homology arms of dInsc were amplified from fly genomic DNA by Phusion® High-Fidelity DNA Polymerase (New England Biolab #M0530S) under optimized conditions. .. The two homology arms were then cloned into pScarlessHD-DsRed (Addgene #64703) plasmid by SOEing PCR, followed by standard heat-shock transformation, colony PCR selection, and Sanger sequencing. .. Embryo microinjection service was provided by Wellgenetics, Taiwan, to inject the gRNA and donor DNA plasmids into fly embryos with Cas9 activity in the germ cell.

    Selection:

    Article Title: A missense mutation in human INSC causes peripheral neuropathy.
    Article Snippet: To clone the donor DNA plasmid, both upstream and downstream homology arms of dInsc were amplified from fly genomic DNA by Phusion® High-Fidelity DNA Polymerase (New England Biolab #M0530S) under optimized conditions. .. The two homology arms were then cloned into pScarlessHD-DsRed (Addgene #64703) plasmid by SOEing PCR, followed by standard heat-shock transformation, colony PCR selection, and Sanger sequencing. .. Embryo microinjection service was provided by Wellgenetics, Taiwan, to inject the gRNA and donor DNA plasmids into fly embryos with Cas9 activity in the germ cell.

    Sequencing:

    Article Title: A missense mutation in human INSC causes peripheral neuropathy.
    Article Snippet: To clone the donor DNA plasmid, both upstream and downstream homology arms of dInsc were amplified from fly genomic DNA by Phusion® High-Fidelity DNA Polymerase (New England Biolab #M0530S) under optimized conditions. .. The two homology arms were then cloned into pScarlessHD-DsRed (Addgene #64703) plasmid by SOEing PCR, followed by standard heat-shock transformation, colony PCR selection, and Sanger sequencing. .. Embryo microinjection service was provided by Wellgenetics, Taiwan, to inject the gRNA and donor DNA plasmids into fly embryos with Cas9 activity in the germ cell.

    Article Title: Dual regulation of the unfolded protein response by IGF2BP3 during ER stress
    Article Snippet: The IGF2BP3 homology arms were inserted into the pMK344 plasmid backbone (Addgene #121179) using SacI and XhoI restriction sites. .. BSD-P2A-mAID sequence was cloned into the plasmid with N-terminal homology arms based on IGF2BP3 from the pMK347 plasmid (Addgene #121181) using SalI and BamI restriction sites resulting in the HDR template. ..

    Article Title: Transcription processes compete with loop extrusion to homogenize promoter and enhancer dynamics
    Article Snippet: .. PCR amplification and Gibson assembly was used to replace the homology arms of plasmid pCRIS-PITChv2-dTAG-Puro (BRD4) (Addgene, #91796) with those for Rad21 , and 1 μg of the vector was cotransfected with 3 μg of plasmid containing Cas9-GFP, a puromycin-resistance marker and a scaffold to encode the gRNA specific to the insertion site (generated by the IGBMC molecular biology platform; sequence of gRNA given in table S1) in 1 million F1 ESCs with Lipofectamine 2000. ..

    other:

    Article Title: Selective RNA sequestration in biomolecular condensates directs cell fate transitions
    Article Snippet: BTAG human iPSCs were maintained on iMatrix-511 (Reprocell) in Stemfit medium (Reprocell) supplemented with 100 ng/ml of bFGF (Peprotech).

    Amplification:

    Article Title: Transcription processes compete with loop extrusion to homogenize promoter and enhancer dynamics
    Article Snippet: .. PCR amplification and Gibson assembly was used to replace the homology arms of plasmid pCRIS-PITChv2-dTAG-Puro (BRD4) (Addgene, #91796) with those for Rad21 , and 1 μg of the vector was cotransfected with 3 μg of plasmid containing Cas9-GFP, a puromycin-resistance marker and a scaffold to encode the gRNA specific to the insertion site (generated by the IGBMC molecular biology platform; sequence of gRNA given in table S1) in 1 million F1 ESCs with Lipofectamine 2000. ..

    Marker:

    Article Title: Transcription processes compete with loop extrusion to homogenize promoter and enhancer dynamics
    Article Snippet: .. PCR amplification and Gibson assembly was used to replace the homology arms of plasmid pCRIS-PITChv2-dTAG-Puro (BRD4) (Addgene, #91796) with those for Rad21 , and 1 μg of the vector was cotransfected with 3 μg of plasmid containing Cas9-GFP, a puromycin-resistance marker and a scaffold to encode the gRNA specific to the insertion site (generated by the IGBMC molecular biology platform; sequence of gRNA given in table S1) in 1 million F1 ESCs with Lipofectamine 2000. ..

    Generated:

    Article Title: Transcription processes compete with loop extrusion to homogenize promoter and enhancer dynamics
    Article Snippet: .. PCR amplification and Gibson assembly was used to replace the homology arms of plasmid pCRIS-PITChv2-dTAG-Puro (BRD4) (Addgene, #91796) with those for Rad21 , and 1 μg of the vector was cotransfected with 3 μg of plasmid containing Cas9-GFP, a puromycin-resistance marker and a scaffold to encode the gRNA specific to the insertion site (generated by the IGBMC molecular biology platform; sequence of gRNA given in table S1) in 1 million F1 ESCs with Lipofectamine 2000. ..



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